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Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages

Received: 22 August 2018     Accepted: 19 September 2018     Published: 10 October 2018
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Abstract

Sugarcane is the most important sugar crop in China, which mainly focuses on the upper stem of the harvested land. The proper regulation of the proportion of internode elongation is the key to determine the yield and sugar. Therefore, it is of great significance to study the mechanism of the dynamic change of elongation between cane joints in order to improve the yield of sugar cane and sucrose. To investigate the biochemical mechanism of stem elongation in sugarcane, stem samples were collected at the pre-elongation stage (9-10 leaves) (Ls1), early elongation stage (12-13 leaves) (Ls2) and rapid elongation stage (15-16 leaves) (Ls3). The change trends in the activities of NAD kinase (NADK), calcium-dependent protein kinase (CDPKs), α-mannosidase, α-galactosidase, β-glucosidase, cellulase, xyloglucan endo-transglycosylase/hydrolase (XTH) and catalase (CAT) were completely consistent, showing rapid elongation stage > early elongation stage > pre-elongation stage, while the activities of β-glucosidase, peroxidase (POD) and α-glucosidase were in opposite, and the activities of calmodulin and β-mannosidase showed the same single-peak trend, and the peak was at early elongation stage. The enzyme activities of β-galactosidase and pectinase did not show significant difference at different stages. The results indicate that the elongation of internodes was closely related to the complex physiological metabolism of sugarcane, and the key enzymes play roles at different time but β-galactosidase and pectinase have little effect on internodes elongation in sugarcane.

Published in American Journal of Plant Biology (Volume 3, Issue 2)
DOI 10.11648/j.ajpb.20180302.12
Page(s) 21-28
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Sugarcane, Stem Elongation, Enzyme Activity, Metabolism

References
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Cite This Article
  • APA Style

    Rongfa Chen, Xing Huang, Lihang Qiu, Yegeng Fan, Ronghua Zhang, et al. (2018). Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages. American Journal of Plant Biology, 3(2), 21-28. https://doi.org/10.11648/j.ajpb.20180302.12

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    ACS Style

    Rongfa Chen; Xing Huang; Lihang Qiu; Yegeng Fan; Ronghua Zhang, et al. Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages. Am. J. Plant Biol. 2018, 3(2), 21-28. doi: 10.11648/j.ajpb.20180302.12

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    AMA Style

    Rongfa Chen, Xing Huang, Lihang Qiu, Yegeng Fan, Ronghua Zhang, et al. Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages. Am J Plant Biol. 2018;3(2):21-28. doi: 10.11648/j.ajpb.20180302.12

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  • @article{10.11648/j.ajpb.20180302.12,
      author = {Rongfa Chen and Xing Huang and Lihang Qiu and Yegeng Fan and Ronghua Zhang and Jinlan Xie and Jianming Wu and Yangrui Li},
      title = {Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages},
      journal = {American Journal of Plant Biology},
      volume = {3},
      number = {2},
      pages = {21-28},
      doi = {10.11648/j.ajpb.20180302.12},
      url = {https://doi.org/10.11648/j.ajpb.20180302.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20180302.12},
      abstract = {Sugarcane is the most important sugar crop in China, which mainly focuses on the upper stem of the harvested land. The proper regulation of the proportion of internode elongation is the key to determine the yield and sugar. Therefore, it is of great significance to study the mechanism of the dynamic change of elongation between cane joints in order to improve the yield of sugar cane and sucrose. To investigate the biochemical mechanism of stem elongation in sugarcane, stem samples were collected at the pre-elongation stage (9-10 leaves) (Ls1), early elongation stage (12-13 leaves) (Ls2) and rapid elongation stage (15-16 leaves) (Ls3). The change trends in the activities of NAD kinase (NADK), calcium-dependent protein kinase (CDPKs), α-mannosidase, α-galactosidase, β-glucosidase, cellulase, xyloglucan endo-transglycosylase/hydrolase (XTH) and catalase (CAT) were completely consistent, showing rapid elongation stage > early elongation stage > pre-elongation stage, while the activities of β-glucosidase, peroxidase (POD) and α-glucosidase were in opposite, and the activities of calmodulin and β-mannosidase showed the same single-peak trend, and the peak was at early elongation stage. The enzyme activities of β-galactosidase and pectinase did not show significant difference at different stages. The results indicate that the elongation of internodes was closely related to the complex physiological metabolism of sugarcane, and the key enzymes play roles at different time but β-galactosidase and pectinase have little effect on internodes elongation in sugarcane.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages
    AU  - Rongfa Chen
    AU  - Xing Huang
    AU  - Lihang Qiu
    AU  - Yegeng Fan
    AU  - Ronghua Zhang
    AU  - Jinlan Xie
    AU  - Jianming Wu
    AU  - Yangrui Li
    Y1  - 2018/10/10
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ajpb.20180302.12
    DO  - 10.11648/j.ajpb.20180302.12
    T2  - American Journal of Plant Biology
    JF  - American Journal of Plant Biology
    JO  - American Journal of Plant Biology
    SP  - 21
    EP  - 28
    PB  - Science Publishing Group
    SN  - 2578-8337
    UR  - https://doi.org/10.11648/j.ajpb.20180302.12
    AB  - Sugarcane is the most important sugar crop in China, which mainly focuses on the upper stem of the harvested land. The proper regulation of the proportion of internode elongation is the key to determine the yield and sugar. Therefore, it is of great significance to study the mechanism of the dynamic change of elongation between cane joints in order to improve the yield of sugar cane and sucrose. To investigate the biochemical mechanism of stem elongation in sugarcane, stem samples were collected at the pre-elongation stage (9-10 leaves) (Ls1), early elongation stage (12-13 leaves) (Ls2) and rapid elongation stage (15-16 leaves) (Ls3). The change trends in the activities of NAD kinase (NADK), calcium-dependent protein kinase (CDPKs), α-mannosidase, α-galactosidase, β-glucosidase, cellulase, xyloglucan endo-transglycosylase/hydrolase (XTH) and catalase (CAT) were completely consistent, showing rapid elongation stage > early elongation stage > pre-elongation stage, while the activities of β-glucosidase, peroxidase (POD) and α-glucosidase were in opposite, and the activities of calmodulin and β-mannosidase showed the same single-peak trend, and the peak was at early elongation stage. The enzyme activities of β-galactosidase and pectinase did not show significant difference at different stages. The results indicate that the elongation of internodes was closely related to the complex physiological metabolism of sugarcane, and the key enzymes play roles at different time but β-galactosidase and pectinase have little effect on internodes elongation in sugarcane.
    VL  - 3
    IS  - 2
    ER  - 

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Author Information
  • Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China

  • Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China

  • Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China

  • Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China

  • Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China

  • Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China

  • Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China

  • Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China

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